The present disclosure is directed to a bacterial shipping and delivery automobile containing the vector or payload as explained herein.
The sequence encoding Mad4 (the entire protein or a fragment thereof) as used in the context on the creation is disclosed in Global software WO2018/236548.
215、在限制性位点序列中，n意指核苷酸可为a、c、g或t；b意指核苷酸可为c、g或t；y意指核苷酸可为c或t；w意指核苷酸可为a或t；r意指核苷酸可为a或g；以及d意指a、g或t。
By “host endogenous molecule” is supposed herein any molecule The natural way made by the host subject matter, in particular by a balanced host matter.
In a specific embodiment, the nucleic acid of fascination encodes a bacteriocin or possibly a lysin, which may be a proteinaceous toxin made by receiver bacteria to kill or inhibit development of other germs. Bacteriocins are classified in various approaches, which include producing pressure, widespread resistance mechanisms, and mechanism of killing.
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fifteen. the strategy In accordance with embodiment 14, whereby stated conditional origin of replication is Lively in 了解更多細節 stated donor bacterial mobile because claimed donor bacterial cell expresses claimed offered protein, peptid, nucleic acid, RNA, molecule or any blend thereof.
By “host exogenous molecule expressed Obviously by other organisms” is supposed herein any molecule which is not produced by the host topic (or by a subject of the exact same species as the host species) but which is of course produced by One more organism, in particular an organism from A different species, from One more gender, from An additional family, from One more course or from A different kingdom.
The present inventors below show, for the first time, that it is feasible to acquire an result in vivo, for instance a therapeutic influence, With all the shipping and delivery of a non-replicative vector to a germs.
in the chosen embodiment, the genetic modification is in human commensal microorganisms encoding a Ro60 ortholog gene. ideally, the Ro60 protein ensuing within the genetic modification reveals decrease homology with human Ro60 peptide compared to the initial protein. if possible the genetic modification is performed while in the DNA sequence corresponding to peptides fragment identified as epitope from the human immune program leading to a weaker or absence of epitope recognition because of the human immune system.
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884 sequences have been found. What's more, it really should be mentioned that when sequencing strains, plasmids can be overlooked with the assembly Should they be modest (by way of example, the pOSAK present in STEC O157 strains), so the quantity of hits may be higher.
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whereby, after delivered into explained focused receiver bacterial mobile, said nucleic acid of interest makes claimed supplied effect on mentioned qualified receiver bacterial mobile when claimed vector is just not replicated in claimed qualified receiver bacterial cell.